Column:

Carbomix H-NP10:8%, 10 µm, 7.8 x 300 mm

Mobile phase:

5 mM H₂SO₄

Flow rate:

0.6 mL/min

Temperature:

37 ^oC

Detection:

UV 210 nm

Injection volume:

20 µL

Sample:

1. 4 μg/mL Maleic acid （马来酸）  
2. 1 mg/mL Malic acid （苹果酸）  
3. 10 μg/mL Fumarate （富马酸）
each in water